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Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
 

Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study

Article DOI: 10.3791/57492-v 08:00 min April 11th, 2018
April 11th, 2018

챕터

요약

Here we describe a method to generate three-dimensional spheroid cultures of human nasal epithelial cells. Spheroids are then stimulated to drive Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)-dependent ion and fluid secretion, quantifying the change in the spheroid luminal size as a proxy for CFTR function.

Tags

Keywords: Nasal Epithelial Cell Spheroids CFTR Studies Cystic Fibrosis Ex Vivo Model Cell Culture Trypsinization Cell Expansion Cell Differentiation Basement Membrane Matrix
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