/

/

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

JoVE 신문
유전학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 유전학

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

DOI:

10.3791/57494-v

•

06:21 min

•

May 07, 2018

•

Seoghyun Lee*¹, Minho Won*², Ran Hee Hwang, Gang Min Hur, Hyunju Ro

¹Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, ²Department of Pharmacology, College of Medicine,Chungnam National University, ³Department of Nursing,Gwangju Women’s University

Chapters

00:05Title
00:45Transient Transfection and Teb Treatment
03:00Depletion of Teb
04:01Results: Modulation of Transgene Expression of the pEUI() Singular Gene Switch using Teb
05:11Conclusion

Summary

자동 번역

Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

Tags

Keywords: Ecdysone Receptor Gene Switch Transgene Expression Tebufenozide HEK 293 Cells EGFP ANKRD13A Transfection Immunoblotting

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Read Article