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基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体

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Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

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基因靶向随机诱变在裂变酵母中选择染色质稳定蛋白酶突变体

DOI:

10.3791/57499-v

•

07:18 min

•

May 15, 2018

•

Hogyu David Seo, Daeyoup Lee

¹Department of Biological Sciences,Korea Advanced Institute of Science and Technology

Chapters

00:04Title
00:37Random Mutagenesis of rpt4+ by Error-prone PCR
01:33Transformation of Fission Yeast by Electroporation
04:01Selection of Heterochromatin-destabilizing Mutants and Checking for False Positives
05:44Results: Random Mutagenesis of Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
06:36Conclusion

Summary

자동 번역

本文介绍了一种用于裂变酵母靶基因随机诱变的详细方法。例如, 我们针对的是rpt4+, 它对19S 蛋白酶体的亚基进行编码, 并筛选出破坏染色质的突变。

Tags

Gene-targeted Random Mutagenesis Heterochromatin-destabilizing Proteasome Mutants Fission Yeast Error-prone PCR Transformation-fusion Construct Electroporation Sorbitol Wash Heterochromatin Formation And Maintenance

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