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鼠脑切片偶联微电极阵列癫痫活性的记录与调控

JoVE 신문
신경과학

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JoVE 신문 신경과학

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays

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鼠脑切片偶联微电极阵列癫痫活性的记录与调控

DOI:

10.3791/57548-v

•

10:24 min

•

May 15, 2018

•

Gabriella Panuccio, Ilaria Colombi, Michela Chiappalone²

¹Department of Neuroscience and Brain Technologies,Istituto Italiano di Tecnologia, ²Rehab Technologies,Istituto Italiano di Tecnologia

Chapters

00:04Title
00:39Preparation of the MEA/Custom Chamber Assembly
01:15Preparation of the Recording Bench
02:52Preparation of the MEA Set-up
04:39MEA Live Mapping
06:33Recording and Electrical Modulation of the Epileptiform Activity
08:49Results: Representative Experiment of Electrical Modulation of Ictogenesis Using Brain Slices Coupled to MEAs
09:21Conclusion

Summary

자동 번역

我们说明如何使用微电极阵列对 4-氨基吡啶诱导的啮齿动物脑切片中的癫痫活性进行记录和电调制。一个自定义记录室保持组织的可行性在长期的实验课。实时电极映射和激发对的选择由自定义图形用户界面执行。

Tags

Keywords: Epileptiform Activity Rodent Brain Slices Microelectrode Arrays Neuromodulation Epilepsy Treatment Brain Circuits MEA ACSF 4AP Hippocampus-cortex Brain Slice Preparation Perfusion Recording Chamber Heating Amplifier

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