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小鼠巨噬细胞吞噬的延时3D 成像
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages
DOI:

07:24 min

October 19, 2018

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Chapters

  • 00:04Title
  • 01:00Resident Mouse Peritoneal Macrophage and Human Red Blood Cell Isolation
  • 04:00Macrophage Plasma Membrane Labeling and Human Red Blood Cell-mouse Immunoglobulin (IgG) Opsonization and Imaging
  • 06:32Results: Representative Time-lapse, 3D-imaging of Phagocytosis
  • 07:02Conclusion

Summary

자동 번역

本文介绍了利用旋转圆盘共聚焦显微镜对小鼠腹腔巨噬细胞单个吞噬事件进行图像的方法。这些协议可以扩展到其他吞噬细胞。

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