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1David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 2Department of Respiratory Medicine, Graduate School of Medicine,The University of Tokyo, 3Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine,University of Southern California, 4Department of Molecular Pathology, Graduate School of Medicine,The University of Tokyo
Chapters
- 00:04Title
- 00:41Induction of EndMT
- 01:58Immunocytochemical Analysis
- 05:02Three-dimensional Collagen Gel Contraction Assay
- 06:09Results: Use of an In Vitro Method to Study Endothelial-mesenchymal Transition Upon TGF-β Treatment
- 07:20Conclusion
Un protocole pour in vitro induction de transition endothéliales-mésenchymateuse (EndMT), qui est utile pour étudier les voies de signalisation cellulaires impliqués dans EndMT, est décrite. Dans ce modèle expérimental, EndMT est induite par le traitement avec le TGF-β dans les cellules endothéliales MS-1.
