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Chapters
- 00:00Title
- 01:24Lysate Preparation
- 03:51Cytoplamic Lysate Preparation from Cultured Human Cells
- 05:15Cytoplasmic Lysate Preparation from Mouse Testis
- 06:53Sucrose Gradient Preparation and Ultracentrifugation
- 09:21Polysome Fractionation and Sample Collection
- 11:45Analysis of the Fractions
- 12:27Results
- 14:14Conclusions
Het algemene doel van Polysoom profiling techniek is analyse van translationeel activiteiten van individuele mRNAs of transcriptome mRNAs tijdens de eiwitsynthese. De methode is belangrijk voor de studie van eiwit synthese verordening, vertaling activering en onderdrukking in gezondheid en meerdere ziekten bij de mens.
