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Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
 

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Article DOI: 10.3791/57629-v • 07:53 min • August 15th, 2018
August 15th, 2018 •
1,4, 1, 1, 1, 1, 3, 1,2, 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

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요약
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Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.

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Keywords: Enteric Glial Cells Isolation Submucosa Lamina Propria Adult Mouse Intestine Dissection Mesentery Longitudinal Muscle Circular Muscle DPBS Razor Blade Cotton Swab Neuroscience Enteric Nervous System
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