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高效生成粘附培养中 Hpsc 的胰腺-二叶多布蛋白 1⁺后后福格内胰腺祖细胞

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Efficient Generation of Pancreas/Duodenum Homeobox Protein 1⁺ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

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高效生成粘附培养中 Hpsc 的胰腺-二叶多布蛋白 1⁺后后福格内胰腺祖细胞

DOI:

10.3791/57641-v

•

08:32 min

•

March 27, 2019

•

Taro Toyoda, Azuma Kimura, Hiromi Tanaka, Kenji Osafune

¹Center for iPS Cell Research and Application (CiRA),Kyoto University

Chapters

00:04Title
00:28Prepare Basement Membrane Matrix-coated Plates
01:15Seed the hPSCs and Initiate Differentiation to Definitive Endoderm
03:57Induce the Differentiation into Definitive Endoderm
05:13Induce the Differentiation into the Primitive Gut Tube
05:47Induce the Differentiation into PDX1⁺ Cells
06:28Results: Analysis of hPSCs and the Generated Pancreas/Duodenum Homeobox Protein 1⁺ Posterior Foregut/Pancreatic Progenitors
08:06Conclusion

Summary

자동 번역

在这里, 我们提出了一个详细的协议, 以区分人类多能干细胞 (Hpsc) 到胰十二指肠蛋白 1⁺ (pdx1⁺) 细胞的产生的非群体类型单层生长的基础上, 胰腺谱系离解的单细胞。该方法适用于同源 hPSC-derived 衍生细胞的产生、遗传操作和筛选。

Tags

Keywords: HPSCs Adhesion Culture Basement Membrane Matrix EDTA Y27632 Cell Dissociation Cell Counting Stage 1A Medium Pancreatic Progenitors

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