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肿瘤免疫和非免疫微环境在小鼠皮下肿瘤中的富集与鉴定

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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

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肿瘤免疫和非免疫微环境在小鼠皮下肿瘤中的富集与鉴定

DOI:

10.3791/57685-v

•

08:32 min

•

June 07, 2018

•

Jared M. Newton*^1,2, Aurelie Hanoteau*¹, Andrew G. Sikora

¹Department of Otolaryngology-Head and Neck Surgery,Baylor College of Medicine, ²Interdepartmental Graduate Program in Translational Biology and Molecular Medicine,Baylor College of Medicine

Chapters

00:04Title
00:55Tumor Harvest and Digestion
03:02Immune and Tumor Cellular Fraction Separation and Labeling
05:57Results: Representative Tumor Component Features
07:27Conclusion

Summary

자동 번역

在这里, 我们描述了一个方法, 以分离和丰富的组成部分, 肿瘤免疫和非免疫微环境的建立皮下肿瘤。该技术允许对肿瘤免疫浸润和非免疫肿瘤分数进行单独分析, 从而可以对肿瘤免疫微环境进行综合表征。

Tags

Keywords: Tumor Immune Microenvironment Tumor Non-immune Microenvironment Immune Oncology Tumor Profiling Tumor Dissociation Tumor Enrichment Tumor Cell Fractionation Tumor Immune Cell Separation Collagenase DNase Density Gradient Medium Subcutaneous Tumors

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