JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 00:54Physical Alignment of Image Splitter Generated Images
- 02:25Alignment of Spectrally Separated Channels by Software Performing Spatial Transformation
- 04:34Single Molecule Imaging of Mitochondrial Membrane Proteins
- 08:32Results: Multi-color Localization of Mammalian Mitochondrial PINK1
- 10:15Conclusion
Vi præsenterer her, en protokol for multi-farve lokalisering af enkelt Membranproteiner i organeller af levende celler. Du kan vedhæfte fluorophores, bruges selvstændig mærkning proteiner. Proteiner, beliggende i forskellige membraner rum af den samme organelle, kan lokaliseres med en nøjagtighed på ~ 18 nm.
Tags
Keywords: Multi-color Localization MicroscopySingle Membrane ProteinsOrganellesLive Mammalian CellsProtein DynamicsSpatiotemporal OrganizationMitochondrial Membrane ProteinsTransfectionSample PreparationMicroscope SetupFluorescent BeadsObjective LensImmersion OilTransmission LightLaserFluorescence ChannelsImage SplitterTIRF MicroscopyTransformation MatrixDual-color ImagesCalibrationUnit ConversionLocalization ManagerPoint Spread Function (PSF)
