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Chapters
- 00:04Title
- 01:35Fabrication of a PDMS Microfluidic Platform
- 03:16Production of Polyacrylamide Oligo-Microspheres
- 06:06Performing DNA Hybridization on the Surface of Polyacrylamide Oligomicrosphere
- 07:06Microsphere-PCR for Amplifying ssDNA
- 08:08Confocal Microscopy Acquisition
- 08:38“Results: Fabrication of Droplet-base Microfluidic Platform and Application of Microspheres for Microsphere-PCR Amplification”
- 10:33Conclusion
Cet ouvrage fournit une méthode pour la fabrication de plateformes microfluidiques axée sur la goutte et l’application des microsphères de polyacrylamide pour l’amplification PCR-microsphères. La méthode PCR-microsphères permet d’obtenir des amplicons ADN monocaténaire sans séparer l’ADN bicaténaire.
