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Dibromomaleimide 二硫化学制备共轭诱导荧光 PEGylated 病毒样粒子
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Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry
DOI:

10:18 min

May 27, 2018

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Chapters

  • 00:05Title
  • 00:41Expression of Qβ
  • 02:51Purification of Qβ
  • 05:51Conjugating DB Compounds on Qβ
  • 08:06Results: Qβ-PEG Can Be Used as a Fluorescence Probe Inside Cells
  • 09:35Conclusion

Summary

자동 번역

在这里, 我们提出一个程序, 荧光 functionalize 二硫化物 Qβ vip 与 dibromomaleimide。我们描述了 Qβ的表达和纯化, dibromomaleimide 功能分子的合成, dibromomaleimide 和 Qβ的共轭反应。生成的黄色荧光共轭粒子可作为细胞内的荧光探针。

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