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小鼠脊髓损伤模型中内源性神经干细胞活化的干细胞检测

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A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

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小鼠脊髓损伤模型中内源性神经干细胞活化的干细胞检测

DOI:

10.3791/57727-v

•

09:08 min

•

September 13, 2018

•

Nishanth Lakshman², Wenjun Xu, Cindi M. Morshead^2,3

¹Institute of Medical Science,University of Toronto, ²Department of Surgery,University of Toronto, ³Institute of Biomaterials and Biomedical Engineering,University of Toronto

Chapters

00:04Title
00:45Minimal Spinal Cord Injury (SCI) Surgery
04:01Spinal Cord Isolation and Neurosphere Culture
07:18Results: Representative Neurosphere Analyses Following Minimal SCI
08:24Conclusion

Summary

자동 번역

在这里, 我们展示了一个最小的脊髓损伤模型的表现, 在一只成年小鼠, 备用的中心运河利基住房内源性神经干细胞。我们展示了如何使用干细胞检测来量化的确定性和原始神经干细胞的活化和迁移后损伤。

Tags

Neural Stem Cells Spinal Cord Injury Neurosphere Assay Endogenous Neural Precursor Cells Regenerative Medicine Mouse Model Surgical Technique

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