Journal
/
생물학
/
视网膜低温切片、全支架和低渗隔离血管毛细血管微血管组织免疫组化的制备
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes
Please note that all translations are automatically generated. Click here for the English version.

视网膜低温切片、全支架和低渗隔离血管毛细血管微血管组织免疫组化的制备

DOI:
10.3791/57733-v

•

10:46 min

•

October 07, 2018

•

, ,
1Department of Clinical Experimental Research,Glostrup Research Institute, 2Department of Clinical Sciences, Division of Experimental Vascular Research,Lund University

Chapters

  • 00:04Title
  • 00:41Cryosection Rat Retinal Tissue Preparation and Immunostaining
  • 03:23Whole Mount Rat Retinal Tissue Preparation and Immunostaining
  • 05:08Hypotonic Isolation of Ray Retinal Tissue Preparation and Immunostaining
  • 08:27Results: Representative Immunohistochemistry Images
  • 10:18Conclusion

Summary

자동 번역

자동 번역

我们展示了三种不同的组织制备技术的免疫组化显示大鼠视网膜微血管毛细血管,即低温切片, 全支架, 和低渗的隔离血管网络。

Tags

Retinal Cryo-sectionsWhole-mountsIsolated VasculatureImmunohistochemistryPericytesEnucleationFormaldehyde FixationCryoprotectionCryosectioningAntibody StainingFluorescent LabelingMicroscopy

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

薄切片进行免疫组化切片的筹备工作

使用激光光镊操纵隔离神经细胞在体外

全安装胚胎肢体皮肤血管的免疫组织化学分析：一个模型系统，研究在胚胎血管分支形态发生

从解离分析准备胚胎和幼体斑马鱼骨肌纤维

视网膜平片准备成像和免疫组化优化方案

精子发生的细胞学分析：Live和固定制剂<em>果蝇</em>睾丸

运动性的时空制图<em>离体</em肠子>准备工作

瑞士改进轧制技术肠组织准备免疫组化和免疫分析

分离块茎原生质体流式细胞仪测量 Endoreduplication 的研究

眼部微血管血管生成前维沃胆球状芽分析

Read Article