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Chapters
- 00:04Title
- 00:58ELP Expression Protocol
- 05:06Cell Encapsulation in 3D Elastin-like Protein Hydrogels
- 07:11Immunocytochemistry of Cells in 3D ELP Hydrogels
- 09:47Results: Purification of Elastin-like Protein and its Application for 3D Hydrogel Cell Encapsulation
- 11:18Conclusion
Recombinant protein-engineered hydrogels are advantageous for 3D cell culture as they allow for complete tunability of the polymer backbone and therefore, the cell microenvironment. Here, we describe the process of recombinant elastin-like protein purification and its application in 3D hydrogel cell encapsulation.
