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Karakterisering van glycoproteïnen met de vouw van de immunoglobuline door middel van röntgendiffractie en biofysische technieken
JoVE 신문
생화학
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JoVE 신문 생화학
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
DOI:

08:58 min

July 05, 2018

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Chapters

  • 00:05Title
  • 00:38Purification of Soluble Glycoprotein from HEK293 Supernatant
  • 02:08Crystallization of Glycoproteins
  • 03:44Soaking Glycoprotein Crystals with its Ligand
  • 04:33Characterization of Fab and Small Molecule Binding to the Glycoprotein
  • 07:34Results: Characterization of Glycoproteins
  • 08:24Conclusion

Summary

자동 번역

Benaderingen voor de biofysische en structurele karakterisatie van glycoproteïnen presenteren wij met de immunoglobuline vouw door biolayer interferometrie, isothermische titratie calorimetrie en röntgendiffractie.

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