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Establishment of a Clonal Culture of Unicellular Conjugating Algae

JoVE 신문
생물학
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JoVE 신문 생물학

Establishment of a Clonal Culture of Unicellular Conjugating Algae

Establishment of a Clonal Culture of Unicellular Conjugating Algae

DOI:

10.3791/57761-v

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10:53 min

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July 14, 2018

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Yuki Tsuchikane, Takashi Hamaji, Katsumi Ota, Syou Kato

¹Department of Chemical and Biological Sciences, Faculty of Science,Japan Women’s University, ²Department of Botany, Graduate School of Science,Kyoto University, ³Katsumi Ota Translation Office, ⁴Wetlands International Japan

Chapters

00:00Title
01:37Collection of water sample containing algae.
02:56Confirmation of alga.
03:46Preparation of a glass capillary pipette from a Pasteur pipette for isolation.
06:29Single-cell isolation by glass capillary pipette.
09:31Results
10:09Conclusion

Summary

자동 번역

In this article, we demonstrate the establishment of clonal cultures of unicellular conjugating algal species collected from a natural field site.

Tags

Keywords: Clonal Culture Unicellular Conjugating Algae Water Sample Single Cell Isolation Sterilization Cultivation Unicellular Conjugatophytes Plankton Net Marsh Paddy Field Microscope Observation Pipette Isolation

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