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Bioengineering

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JoVE Journal Bioengineering
Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
 

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Article DOI: 10.3791/57763-v • 06:51 min • August 10th, 2018
August 10th, 2018 •
*1, *1, 2, 2, 2, 1, 3, 2, 1
1Department of Bioengineering, The University of Texas at Arlington, 2Department of Medicine (Cardiology) and Bioengineering, UCLA, 3College of Health Science and Environmental Engineering, Shenzhen Technology University
* These authors contributed equally

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요약
Automatic Translation

Here, we present a protocol to visualize developing hearts in zebrafish in 4-Dimensions (4-D). 4-D imaging, via light-sheet fluorescence microscopy (LSFM), takes 3-Dimensional (3-D) images over time, to reconstruct developing hearts. We show qualitatively and quantitatively that shear stress activates endocardial Notch signaling during chamber development, which promotes cardiac trabeculation.

Tags

Light-sheet Fluorescence Microscopy 4-dimensional Images Modulating Shear Stress Developing Zebrafish Heart Cardiac Mechanobiology Notch Signaling 4D Light Sheet Microscope Photo Bleaching Photo Damage Computational Algorithm Beating Zebrafish Heart Congenital Cardiac Diseases Invivo Sequential Visualization Diagnostic Tool Three Dimensions Over Time System Creation Accurate Alignment Breeding Tanks Zebrafish Embryos Morpholino Oligo Inhibition Of Trabeculation Agarose Gel Preparation
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