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Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
DOI:

08:03 min

July 12, 2018

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Chapters

  • 00:04Title
  • 00:40Inoculating Zebrafish with V. cholerae
  • 01:49Collecting Intestines and Assessing Bacterial Colonization
  • 04:27Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
  • 06:43Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
  • 07:35Conclusion

Summary

자동 번역

Zebrafish are a natural Vibrio cholerae host and can be used to recapitulate and study the entire infectious cycle from colonization to transmission. Here, we demonstrate how to assess V. cholerae colonization levels and quantify diarrhea in zebrafish.

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