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Kwantificeren van de kolonisatie van Vibrio cholerae en diarree in het volwassen Zebrafish Model
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
DOI:

08:03 min

July 12, 2018

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Chapters

  • 00:04Title
  • 00:40Inoculating Zebrafish with V. cholerae
  • 01:49Collecting Intestines and Assessing Bacterial Colonization
  • 04:27Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
  • 06:43Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
  • 07:35Conclusion

Summary

자동 번역

Zebravis zijn een natuurlijke Vibrio cholerae host en kan worden gebruikt om te recapituleren en de studie van de gehele besmettelijke cyclus van kolonisatie tot transmissie. Hier laten we zien hoe V. cholerae kolonisatie niveaus beoordelen en kwantificeren van diarree in zebrafish.

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