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细胞法检测酶替代疗法中细胞吸收的抗体

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

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细胞法检测酶替代疗法中细胞吸收的抗体

DOI:

10.3791/57777-v

•

07:52 min

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September 10, 2018

•

Ruby Cheung, Gregory W. deHart, Lynne Jesaitis, Stephen J. Zoog, Andrew C. Melton

¹BioMarin Pharmaceutical Inc

Chapters

00:04Title
00:56Cell Plating and Sample Preparation
03:43Sample Addition and Cell Viability Staining
05:44Results: Representative Neutralizing Antibody (NAb) Detection by Enzyme Replacement Therapy (ERT)
07:09Conclusion

Summary

자동 번역

在这里, 我们提出了一种基于细胞的流式细胞仪方法来检测中和抗体或其他因素, 干扰细胞吸收酶替代疗法在人类基质, 如脑脊髓液 (CSF) 或人血清。

Tags

Keywords: Antibodies Neutralizing Enzyme Replacement Therapy Cell-based Assay Immunogenicity Bioanalytical Drug Safety Efficacy Pharmacokinetics Pharmacodynamics Jurkat Cells Mannose-6-phosphate Receptor Flow Cytometry ERT Beads Magnetic Separation Rotary Shaker

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