Journal
/
면역학 및 감염병학
/
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

DOI:
10.3791/57780-v

•

12:36 min

•

June 26, 2018

•

, , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2OMNI Biomarkers, Development Sciences,Genentech, 3Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Chapters

  • 00:04Title
  • 01:10Stimulation and Processing of Peripheral Whole Blood
  • 05:27Barcode of Lysed/Fixed Blood Cells
  • 09:44Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
  • 11:50Conclusion

Summary

자동 번역

자동 번역

Here we describe a single-cell proteomic approach to evaluate immune phenotypic and functional (intracellular cytokine induction) alterations in peripheral whole blood samples, analyzed via mass cytometry.

Tags

Keywords: Single-cell AnalysisImmunophenotypeCytokine ProductionPeripheral Whole BloodMass CytometryAutoimmunityCell FrequencyCytokine ProductionSystems ImmunologyAutoimmune DiseaseSLEWhole Blood ProcessingRuxolitinibLyse/fix BufferCell RecoveryAntibody Staining

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/57780?article_type=v

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
check_url/kr/57780?article_type=v

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
check_url/kr/57780?article_type=v

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
check_url/kr/57780?article_type=v

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
check_url/kr/57780?article_type=v

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
check_url/kr/57780?article_type=v

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
check_url/kr/57780?article_type=v

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
check_url/kr/57780?article_type=v

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood
check_url/kr/57780?article_type=v

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
check_url/kr/57780?article_type=v

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Read Article