/

/

一种简便有效的成人皮肤组织中人角质形成细胞的分离方法

JoVE 신문
발생학
Author Produced

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 발생학

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Please note that all translations are automatically generated. Click here for the English version.

一种简便有效的成人皮肤组织中人角质形成细胞的分离方法

DOI:

10.3791/57784-v

•

06:17 min

•

August 25, 2018

•

Zhenan Liu, Jie Wen², Xue Leng, Qian Zhou, Changkuo Zhou, Huaqiang Zhao, Xunwei Wu²

¹Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, ²Suzhou Institute of Shandong University, ³Department of Urology,Qilu Hospital of Shandong University

Chapters

00:00Title
00:20Skin Tissue Pretreatment
01:07Digestion of skin tissue
02:11Collecting and culturing primary cells
03:42Cell Passage
04:46Representative results
06:06Conclusion

Summary

자동 번역

在这里, 我们提出了一个协议, 以有效地隔离主要的人角质细胞从成人皮肤组织。该方法利用接种培养基中的岩石抑制剂 Y-27632, 将表皮细胞与真皮细胞自发分离, 从而简化了常规程序。

Tags

Primary Human Keratinocytes Isolation Culture Simplified Method Efficient Epidermal Cells Laboratory Applications Clinical Applications Homogenization Enzymatic Digestion Trypsin DNase I Cell Strainer Cell Culture ROCK Inhibitor Keratinocyte Medium Passaging

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

从人类骨骼肌成肌原细胞和成纤维细胞的分离和定量免疫细胞化学表征

转染，选择和人类诱导多能干细胞集落采摘TALEN靶向用GFP基因进入AAVS1安全港

为内胚层细胞从人类胚胎干细胞系生成的分离纯化一种快速有效的方法

后生转换为安全和简单的方法来获得成人皮肤成纤维细胞胰岛素分泌细胞

从背小鼠皮肤分离毛囊干细胞和表皮角质形成细胞

一个有效的方法获取反分化脂肪细胞

从人胎盘组织源分离和间充质干扩张/基质细胞

成人皮肤角质形成细胞的生成与培养

人成体和胎心标本中原发性心外膜细胞的分离培养

一种直接而简单的方法，用于评估从胚胎到成人的果蝇黑色素的生存能力

Read Article