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Chemistry

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Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
 

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Article DOI: 10.3791/57789-v • 08:15 min • July 16th, 2018
July 16th, 2018 •
1, 1, 1, 1
1Department of Chemistry and Chemical Engineering, Chalmers University of Technology

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요약
Automatic Translation

We present a technique for contactless micromanipulation of vesicles, using localized calcium ion gradients. Microinjection of a calcium ion solution, in the vicinity of a giant lipid vesicle, is utilized to remodel the lipid membrane, resulting in the production of membrane tubular protrusions.

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Membrane Remodeling Giant Vesicles Calcium Ion Gradients Lipid Membrane Contactless Stimulation Visualization Micro-environment Lipids In Chloroform Lipid Concentration Metal Foil-wrapped Sample Vial Evaporation Process Dry Lipid Film Phosphate-buffered Saline Solution Glycerol Giant Unilamellar Vesicle Multilamellar Vesicle GUV-MLV
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