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间歇酵母 2-混合筛序列数据的信息化分析

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Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

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间歇酵母 2-混合筛序列数据的信息化分析

DOI:

10.3791/57802-v

•

09:14 min

•

June 28, 2018

•

Venkatramanan Krishnamani, Tabitha A. Peterson, Robert C. Piper, Mark A. Stamnes

¹Molecular Physiology and Biophysics,University of Iowa

Chapters

00:04Title
00:50Mapping Fastq Files
02:14Bioinformatics Processing Using DEEPN Software
06:08Verification of Candidates Identified by DEEPN
08:01Conclusion

Summary

자동 번역

酵母菌群的深度测序为阳性酵母 2-混合相互作用可能产生大量的信息关于相互作用的伙伴蛋白。在这里, 我们描述了具体的生物信息学工具的操作和定制的更新软件来分析这些屏幕的序列数据。

Tags

Keywords: Informatic Analysis Sequence Data Batch Yeast 2-hybrid Screens Protein Interactomes Bioinformatics Processing User-friendly Graphic User Interface Molecular Biologists Cell Biologists Biochemists MAPster Software DEEPN Analysis HISAT2 Multi-threading Reference DNA Genome Y2H Prey Library Inserts Mapped Files Unmapped Reads

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