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Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Chapters
- 00:04Title
- 01:01Adipose Stromal Vascular Fraction Isolation
- 03:07Isolation of Adipose Tissue Endothelial Cells
- 06:08Induction of Adipogenesis in Isolated Endothelial Cells
- 07:13Results: CD31+CD34+ have Endothelial Phenotype and Display Depot- and Subject-specific Responses to Adipogenic Induction
- 08:57Conclusion
The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed.
