Journal
/
생화학
/
线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
Please note that all translations are automatically generated. Click here for the English version.

线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

DOI:
10.3791/57807-v

•

09:35 min

•

October 15, 2018

•

, ,
1Department of Health Science, School of Sports & Health Science,Daito Bunka University, 2Department of Molecular Life Science, School of Medicine,Tokai University, 3Department of Health Management, School of Health Study,Tokai University

Chapters

  • 00:04Title
  • 00:37Caenorhabditis elegans Synchronized Culture
  • 03:21Extraction of Cellular Fraction from C. elegans
  • 05:35Lactate, Pyruvate and Protein Assays
  • 07:32Results: Efficiency of Lactate and Pyruvate Colorimetric Assays After Protein Precipitation of C. elegans
  • 08:26Conclusion

Summary

자동 번역

자동 번역

本文介绍了一种改良的小尺度萃取和比色法测定乳酸和丙酮酸在线虫中的含量.使用商用试剂盒时, 其灵敏度和准确度的技术发展非常重要。在萃取中蛋白质沉淀是定量测定细胞内代谢产物的关键步骤。

Tags

Keywords: Colorimetric AssayLactatePyruvateCaenorhabditis ElegansNematodeProtein PrecipitationHomogenizationE. Coli OP50NGM Agar PlatesGravid HermaphroditesEgg IsolationL1 LarvaeSynchronous CultureColorimetric AnalysisBiochemical Methodology

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/57807?article_type=v

共价结合的抗体，以纤维素纸光盘及其裸眼比色法免疫分析应用
check_url/kr/57807?article_type=v

自动声胶肽受体激动剂的效价测定测定的连续稀释
check_url/kr/57807?article_type=v

一种简单的高通量化学筛选方法在秀丽线虫中的应用
check_url/kr/57807?article_type=v

测定植物铁含量的比色法
check_url/kr/57807?article_type=v

映射代谢: 直接在组织中监测乳酸脱氢酶活性
check_url/kr/57807?article_type=v

分光光度法测定蓝藻Synechocystis中的蛋白含量
check_url/kr/57807?article_type=v

基于板块的秀丽线虫规模化栽培: 糖尿病代谢变化研究的样品制备
check_url/kr/57807?article_type=v

核糖体组蛋白的体外泛化和二次基质测定
check_url/kr/57807?article_type=v

果蝇梅拉诺加斯特的锡酸盐合成酶活性的色度测定
check_url/kr/57807?article_type=v

Caenorhabditis elegans中 2-阿拉希多诺诺基甘油定量的分解标准合成

Read Article