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Biochemistry

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Expression and Purification of Mammalian Bestrophin Ion Channels
 

Expression and Purification of Mammalian Bestrophin Ion Channels

Article DOI: 10.3791/57832-v 08:12 min August 2nd, 2018
August 2nd, 2018

챕터

요약

The purification of ion channels is often challenging, but once achieved, it can potentially allow in vitro investigations of the functions and structures of the channels. Here, we describe the stepwise procedures for the expression and purification of mammalian bestrophin proteins, a family of Ca2+-activated Cl- channels.

Tags

Keywords: Mammalian Bestrophin Ion Channels Protein Expression Purification HEK293F Cells Baculovirus Sodium Butyrate Cell Density Cell Viability Protein Purification
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