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Journal / Immunology and Infection / 果蝇蛹中炎症细胞动力学的长期体内追踪…
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Immunology and Infection

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JoVE Journal Immunology and Infection
果蝇蛹中炎症细胞动力学的长期体内追踪
 
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果蝇蛹中炎症细胞动力学的长期体内追踪

Article DOI: 10.3791/57871-v • 09:26 min • June 14th, 2018
June 14th, 2018 •
1,2, 1, 3, 1,4
1School of Biochemistry, Biomedical Sciences, University of Bristol, 2School of Cellular and Molecular Medicine, Biomedical Sciences, University of Bristol, 3MRC Centre for Inflammation Research, University of Edinburgh, Queens Medical Research Institute, 4School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences, University of Bristol

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在这里, 我们提出了一个实时成像伤口修复的协议和相关的炎症反应在高时空分辨率的体内。该方法利用果蝇发育的蛹阶段, 实现对特定细胞种群的长期成像和跟踪, 并与有效的 rna 干扰介导的基因失活相兼容。

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免疫学和感染 问题 136 伤口愈合 炎症 先天免疫,果蝇,果蝇蛹 全血细胞 细胞迁移 活成像 激光消融 共焦显微术 photoconversion
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