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1Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine,Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences,Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine,Stanford University
Chapters
- 00:04Title
- 00:34Preparations and Culture of RBL Cells for Live Cell Microscopy
- 01:52Live Cell Microscopy of Exocytosis
- 03:43Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
- 04:17Conclusion
Hier detail we een methode om levende cellen beeldvorming van gereglementeerde exocytose. Deze methode maakt gebruik van FITC-dextran, die hoopt zich op in lysosoom-gerelateerde organellen, als verslaggever. Deze eenvoudige methode kan ook onderscheid te maken tussen de verschillende modi van gereglementeerde exocytose in cellen die moeilijk zijn te genetisch manipuleren.
