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1Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine,Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences,Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine,Stanford University
Chapters
- 00:04Title
- 00:34Preparations and Culture of RBL Cells for Live Cell Microscopy
- 01:52Live Cell Microscopy of Exocytosis
- 03:43Results: FITC-dextran May Act as a Reporter for Regulated Exocytosis
- 04:17Conclusion
Ici nous détailler une méthode d’imagerie de cellules vivantes d’exocytose régulée. Cette méthode utilise la FITC-dextran, qui s’accumule dans les organites liés lysosome, comme journaliste. Cette méthode simple permet également de faire la distinction entre les différents modes d’exocytose régulée dans les cellules qui sont difficiles à manipuler génétiquement.
