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Immunology and Infection

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JoVE Journal Immunology and Infection
Karakterisering af humane monocyt delmængder af fuldblod Flow flowcytometri analyse
 
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Karakterisering af humane monocyt delmængder af fuldblod Flow flowcytometri analyse

Article DOI: 10.3791/57941-v • 09:12 min • October 17th, 2018
October 17th, 2018 •
1,2, 1,2, 3, 4, *1,2, *1,2
1Department of Surgery, Vascular Biology Research Centre, Westmead Hospital, 2Westmead Clinical School, Department of Surgery, The University of Sydney, 3Westmead Research Hub, Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research, Westmead Hospital
* These authors contributed equally

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Her præsenterer vi en protokol for kendetegner monocyt delmængder af fuldblod flowcytometri. Dette omfatter skitserer, hvordan gate delmængder og vurdere deres udtryk for celleoverflademarkører og giver et eksempel på vurdering af udtryk for M1 (inflammatoriske) og M2 markører (anti-inflammatoriske).

Tags

Immunologi og infektion spørgsmål 140 monocyt immunologi flowcytometri betændelse monocyt gating monocyt markør udtryk makrofager åreforkalkning
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