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用快速 Bioorthogonal 反应生成抗体-药物共轭物的非规范氨基酸的遗传编码

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Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

Please note that all translations are automatically generated. Click here for the English version.

用快速 Bioorthogonal 反应生成抗体-药物共轭物的非规范氨基酸的遗传编码

DOI:

10.3791/58066-v

•

11:02 min

•

September 14, 2018

•

Benjamí Oller-Salvia

¹Medical Research Council Laboratory of Molecular Biology

Chapters

00:04Title
00:30Produce and Characterize the Antibody
03:47Conjugate the Antibody and Characterize the Antibody-Drug Conjugates (ADC)
06:38Assay Stability of the Dihydropyridazine Linkage in Trastuzumab(TAMRA)₂ in Serum
07:46Assess the Cytotoxicity of the ADC
09:11Results: Antibody-Drug Conjugate is Stable, Potent, and Selective
10:31Conclusion

Summary

자동 번역

将赖氨酸的 cyclopropene 衍生物加入抗体, 就能使四嗪类分子的特异性、快速和有效的联系产生抗体-药物共轭。

Tags

Genetic Encoding Non-canonical Amino Acid Antibody-drug Conjugates Bioorthogonal Reaction Protein Modification Antibody Production CpK HEK Cells Protein A Purification FPLC Hydrophobic Interaction Chromatography

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