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In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
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In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy

DOI:
10.3791/58083-v

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09:45 min

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August 24, 2018

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1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine,University of California, Davis

Chapters

  • 00:04Title
  • 00:34Neutrophil Isolation
  • 03:03Live Neutrophil Imaging and Neutrophil Extracellular Trap (NET) Detection and Quantification
  • 07:18Results: Representative Canine Neutrophil Imaging and NET Detection
  • 08:55Conclusion

Summary

자동 번역

자동 번역

We describe methods to isolate canine neutrophils from whole blood and visualize NET formation in live neutrophils using fluorescence microscopy. Also described are protocols to quantify NET formation and citrullinated histone H3 (citH3) expression using immunofluorescence microscopy.

Tags

In VitroCanineNeutrophilExtracellular TrapFormationFluorescence MicroscopyNETPeroxidaseCitrullinated Histone H3BloodJugular VeinAnticoagulantDextranDensity Gradient CentrifugationErythrocytesPolymorphonuclear CellsCell Culture BufferTrypan BluePoly-L-lysineFluorescence Microscopy

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