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Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
 

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Article DOI: 10.3791/58099-v • 09:14 min • August 12th, 2018
August 12th, 2018 •
*1,2, *1,2, 3, 1,2
1Peripheral Neuropathy Research Center, College of Medicine, Dong-A University, 2Department of Biochemistry, College of Medicine, Dong-A University, 3Aging Institute, Department of Medicine, University of Pittsburgh School of Medicine
* These authors contributed equally

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요약
Automatic Translation

Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.

Tags

Mitophagy Flow Cytometry PH-dependent Fluorescent Reporter Mt-Keima Sensitive Measurement Cellular Mitophagy Activity HeLa Cells CCCP Treatment Mitochondrial Uncoupler Living Cells Additional Staining Mechanism And Physiological Role Of Mitophagy Mt-Keima Lentivirus HEK293T Cells Transfection Reagent Viral Particles
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