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Chapters
- 00:04Title
- 00:51Patterned Sapphire Disc Preparation, Cell Culture, and Imaging
- 03:16Cryo-immobilization and Freeze Substitution (FS)
- 05:24Resin Embedding and Patterned Sapphire Disc Removal from the Polymerized Resin Block
- 06:43Ultrathin Section Preparation and Imaging via Transmission Electron Microscopy (TEM)
- 08:06Results: Tracking and Imaging of Viral Protein-associated Structures via Correlative Light Electron Microscopy (CLEM)
- 08:47Conclusion
En korrelat ljus elektronmikroskopi (CLEM) metod tillämpas på bild virus-inducerad intracellulära strukturer via elektronmikroskopi (EM) i celler som tidigare valts av ljusmikroskop (LM). LM och EM kombineras som en hybrid imaging strategi att uppnå en integrerad vy av virus-host interaktioner.
