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Chapters
- 00:04Title
- 00:34Pituitary Dissection and Cell Dissociation
- 05:58Results: Dissociated Medaka Pituitary Cell Viability in Primary Cell Culture
- 07:07Conclusion
Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful results.
