JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Title
- 00:28Caffeine Extraction in Cell Suspension of C. arabica L.
- 00:55Conditions for the Assessment of Caffeine by Thin Layer Chromatography (TLC) Denistometry
- 01:29Ribonucleic Acid (RNA) Isolation
- 02:55Incubation of RNA Transcript with Deoxyribonuclease (DNase)
- 03:59Complementary Deoxyribonucleic Acid (cDNA) Synthesis
- 05:03Real-Time Quantitative PCR (qPCR) to Amplify the Gene CCS1
- 05:43Total Protein of Cell Suspensions of C. arabica L.
- 06:26Enzymatic Assay for Caffeine Synthase
- 07:39Results: Enzymatic Activity of Caffeine Synthase Gene Evaluated via qPCR
- 08:30Conclusion
