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1Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, 3Center for Nano Science,Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen
Chapters
- 00:04Title
- 00:39Immobilization of Proteins via Functional Silane Coupling Agents
- 04:42Atomic Force Microscopy Based Single Molecule Force Spectroscopy
- 08:38Results: Single Force Spectroscopy
- 10:07Conclusion
Denne protokol beskriver den kovalente immobilisering af proteiner med en heterobifunctional silan kobling agent til siliciumoxid overflader designet til atomic force mikroskopi baseret enkelt molekyle kraft spektroskopi som er eksemplificeret ved den samspillet mellem RrgA (pilus-1 spids adhesin af S. pneumoniae) med fibronektin.
