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Chapters
- 00:04Title
- 00:33Prepare Infiltration Suspension
- 02:11Perform Vacuum Infiltration
- 03:56Growth and Harvest of the Infiltrated Leaves and Pressurized Solvent Extraction
- 05:38Basic Ion Exchange Resin Treatment for Removal of Chlorophylls
- 06:58Results: Infiltration Coverage and Isolated Triterpene Product
- 07:49Conclusion
Forbigående Heterolog ekspression af biosyntetiske enzymer i Nicotiana benthamiana blade kan aflede endogene forsyninger af 2,3-oxidosqualene mod produktion af nye værdifulde triterpene produkter. Heri er beskrevet en detaljeret protokol for hurtig (5 dage) præparativ-skala produktion af Triterpener og analoger udnytte denne kraftfulde plante-baserede platform.
Tags
Transient ExpressionNicotiana BenthamianaTriterpene ProductionPreparative ScaleAgrobacterium TumefaciensCo-infiltrationVacuum InfiltrationMMA BufferOD 600Preparative-scale AccessStructural CharacterizationMedicinally Relevant Biological ActivitySelective LB AgarSelective LB MediaCentrifugationResuspensionInfiltration Suspension
