Journal
/
생체공학
/
Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells
JoVE 신문
생체공학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생체공학
Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

DOI:
10.3791/58176-v

•

08:35 min

•

August 24, 2018

•

, , ,
1Department of Bioengineering,University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute,University of California, Los Angeles

Chapters

  • 00:04Title
  • 00:31Hydrogel Crosslinking and Cell Encapsulation
  • 03:11Hydrogel Culture Single Cell Extraction for Flow Cytometry and Cryopreservation for Sectioning
  • 06:10Results: Hyaluronic Acid-Based 3D Patient-Derived Glioblastoma Cell Hydrogel Cultures
  • 07:55Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol for three-dimensional culture of patient-derived glioblastoma cells within orthogonally tunable biomaterials designed to mimic the brain matrix. This approach provides an in vitro, experimental platform that maintains many characteristics of in vivo glioblastoma cells typically lost in culture.

Tags

Hyaluronic AcidHydrogels3D Cell CultureGlioblastomaExtracellular MatrixPatient-derived CellsCell EncapsulationPEG-MalSilicone Rubber MoldsCell DissociationCell SuspensionGelation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Micropatterned Surfaces to Study Hyaluronic Acid Interactions with Cancer Cells

Fabrication of Micropatterned Hydrogels for Neural Culture Systems using Dynamic Mask Projection Photolithography

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System

Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Read Article