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高通量自动 Microbioreactor 系统在 CHO 细胞模型 IgG1 生产中的应用

JoVE 신문
생체공학

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JoVE 신문 생체공학

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

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高通量自动 Microbioreactor 系统在 CHO 细胞模型 IgG1 生产中的应用

DOI:

10.3791/58231-v

•

08:15 min

•

September 28, 2018

•

Sai Rashmika Velugula-Yellela, Casey Kohnhorst, David N. Powers, Nicholas Trunfio, Anneliese Faustino, Phillip Angart, Erica Berilla, Talia Faison, Cyrus Agarabi

¹Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II,U.S. Food and Drug Administration

Chapters

00:04Title
00:46Seed Train Expansion and Automated Microbioreactor System Preparation
02:55Automated Microbioreactor Software Initiation
05:15System Shutdown
05:58Cell Culture Harvest and IgG Titer Measurement
07:04Results: Representative High-Throughput Automated Microbioreactor Analyses
07:38Conclusion

Summary

자동 번역

提出了一种 microbioreactor 系统在不同条件下并行运行48平行细胞培养的详细协议。介绍了细胞培养过程、收获和随后的抗体效价分析。

Tags

Keywords: High-throughput Automated Microbioreactor System IgG1 Production CHO Cells Cell Line Characterization Process Optimization Shake Flasks Automation Small-scale Studies Cell Culture Subculturing Cell Counter Microbioreactor Software Temperature Control Dissolved Oxygen PH Monitoring

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