Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

Wen-Ta Li; Bang-Yeh Liou; Wei-Cheng Yang; Meng-Hsien Chen; Hui-Wen Chang; Hue-Ying Chiou; Victor Fei Pang; Chian-Ren Jeng

doi:10.3791/58232

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환경과학

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Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

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JoVE 신문 환경과학

Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

DOI:

10.3791/58232-v

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07:05 min

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October 04, 2018

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Wen-Ta Li, Bang-Yeh Liou, Wei-Cheng Yang, Meng-Hsien Chen, Hui-Wen Chang², Hue-Ying Chiou, Victor Fei Pang², Chian-Ren Jeng²

¹Graduate Institute of Molecular and Comparative Pathobiology,National Taiwan University, ²School of Veterinary Medicine,National Taiwan University, ³Department of Oceanography and Asia-Pacific Ocean Research Center,National Sun Yat-sen University, ⁴Graduate Institute of Veterinary Pathobiology,National Chung Hsing University

Chapters

00:04Title
00:57Autometallography Analysis Tissue Sample Preparation
03:48Semi-Quantitative AMG Analysis and Cetacean Histological Ag Assay (CHAA) by Regression
05:54Results: Representative Histological Images of AMG Positive Signals in the Liver and Kidney Tissues of Stranded Cetaceans
06:35Conclusion

Summary

자동 번역

A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.

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Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

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