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高效可扩展的人多潜能干细胞临床相容角膜缘上皮干细胞的定向分化

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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

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高效可扩展的人多潜能干细胞临床相容角膜缘上皮干细胞的定向分化

DOI:

10.3791/58279-v

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10:07 min

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October 24, 2018

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Heidi Hongisto*¹, Meri Vattulainen*¹, Tanja Ilmarinen, Alexandra Mikhailova, Heli Skottman

¹BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, ²Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences,University of Tampere

Chapters

00:04Title
00:41Step I: Corneal Induction in Suspension Culture
03:07Step II: Corneal Differentiation in Adherent Culture
04:33Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
06:08Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
07:25Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells
09:31Conclusion

Summary

자동 번역

本协议介绍了一种简单的两步法在异种和无细胞培养条件下, 从人多潜能干细胞中分化角膜缘上皮干细胞的方法。这里介绍的细胞培养方法可实现经济高效的大规模生产, 适用于角膜细胞治疗使用的临床质量细胞。

Tags

Keywords: Corneal Limbal Epithelial Stem Cells Human Pluripotent Stem Cells Feeder-free Embryoid Body Formation Basal Induction Medium SB-505124 Human Basic Fibroblast Growth Factor Limbal Stem Cell Deficiency Corneal Cell Replacement Therapy

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