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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
 

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Article DOI: 10.3791/58283-v 09:14 min August 20th, 2018
August 20th, 2018

챕터

요약

Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells.

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Keywords: Baculovirus Expression Vectors Secreted Plant Proteins Insect Cells Protein Structure Determination Recombinant Proteins DNA Cloning DNA Sequencing Bacterial Transformation Bacmid DNA Sf9 Cells
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