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温度控制384孔板中用 Ca 敏感荧光染料测定人 iPSC 的心肌细胞较多的收缩

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Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates

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温度控制384孔板中用 Ca 敏感荧光染料测定人 iPSC 的心肌细胞较多的收缩

DOI:

10.3791/58290-v

•

07:42 min

•

October 18, 2018

•

Camille Forny, Romain Sube, Eric A. Ertel

¹Department of Pharmacology and Preclinical Development,Idorsia Pharmaceuticals Ltd

Chapters

00:04Title
00:33Cell Plates Preparation
02:26Assay Preparation
03:43Data Acquisition and Analysis
05:52Results: Effects of Cardiac Ion Channel Blockers on Cardiomyocytes
07:09Conclusion

Summary

자동 번역

人诱导的多潜能干细胞自发收缩心肌细胞较多是人类心脏生理学和药理学的有用模型。在这里, 我们提出了一个高通量的筛选系统, 以量化的影响, 外源化合物对跳动频率, 使用 Ca 敏感荧光染料和温度控制成像多孔板读取器。

Tags

Human-iPSC-derived Cardiomyocyte Cardiac Safety Pharmacology Temperature-controlled 384-well Plates Ca-sensitive Fluorescent Dye Syncytia Contractions Cell Culture Maintenance Medium Compound Plates

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