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Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

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Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

DOI:

10.3791/58303-v

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07:36 min

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September 26, 2018

•

Vincenzo Marzolla, Andrea Armani, Caterina Mammi, Alessandra Feraco, Massimiliano Caprio²

¹Laboratory of Cardiovascular Endocrinology,IRCCS San Raffaele Pisana, Rome, Italy, ²Department of Human Sciences and Promotion of the Quality of Life,San Raffaele Roma Open University, Rome, Italy

Chapters

00:04Title
00:45Osmotic Pump Filling and Implantation
02:38Heart Dissection
04:03Aortic Root Sectioning and Oil Red O Neutral Fat Staining
05:47Results: Evaluation of the Aortic Root Phenotype in Aldosterone-induced Atherosclerosis in Apolipoprotein-E Knockout (ApoE ^-/-) Mice
06:28Conclusion

Summary

자동 번역

We describe a protocol to induce atherosclerosis in the aortic root of ApoE^-/- mice fed with an atherogenic diet, through a continuous release of aldosterone. Methods to characterize plaque composition are also described.

Tags

Keywords: Atherosclerosis Apolipoprotein E-deficient Mice Mineralocorticoid Receptors Aldosterone Mini-pump Implantation Atherosclerotic Plaque Induction Perfusion

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