/

/

种系线虫与胸苷模拟教育的细胞周期分析

JoVE 신문
발생학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 발생학

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Please note that all translations are automatically generated. Click here for the English version.

种系线虫与胸苷模拟教育的细胞周期分析

DOI:

10.3791/58339-v

•

06:58 min

•

October 22, 2018

•

Zuzana Kocsisova², Ariz Mohammad, Kerry Kornfeld, Tim Schedl

¹Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, ²Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Chapters

00:04Title
00:505-ethynyl-2′-deoxyuridine (EdU)-labeled Bacteria Preparation and C. elegans Feeding
02:46Germline Dissection, Fixation, Rehydration, and EdU Click Reaction/Detection
05:09Results: Representative C. elegans Cell Cycle Analyses
06:20Conclusion

Summary

자동 번역

本文描述了一种基于成像的方法, 可用于识别 S 相和分析种系中的细胞周期动力学. 此方法不需要转基因, 并且与免疫荧光染色兼容。

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

生殖系化学毒性的利用线虫综合评估<em>秀丽隐杆线虫</em

通过在胚胎梅克尔软骨Zebrabow克隆细胞分析软骨插层和定向扩散的可视化

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

在Active ERK的时空分析<em>℃。线虫</em>生殖系

C. 线虫小肠作为细胞间腔形态发生和体内极化膜生物单细胞水平的模型: 抗体染色、rna 干扰功能分析和成像标记

rna 干扰和热休克诱导转录因子表达在重编程生殖细胞到神经元中的应用C. 线虫

细胞聚集法评价果蝇切口与反式配体的结合及其对顺式配体的抑制作用

Immunolabeled, 澄清人胎盘长柔毛血管网络的三维渲染与分析

秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

小鼠皮肤角质细胞的分离和染色,用于细胞周期特定分析细胞蛋白表达的量度细胞测定

Read Article