Journal
/
면역학 및 감염병학
/
Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

DOI:
10.3791/58341-v

•

09:12 min

•

November 14, 2018

•

, , , , , ,
1Department of Psychiatry,Columbia University, 2Department of Pathology & Cell Biology,Columbia University Medical Center, 3Department of Psychiatry, Division of Molecular Imaging and Neuropathology,New York State Psychiatric Institute, 4EB Sciences

Chapters

  • 00:04Title
  • 00:48Tissue Preparation and Protein Extraction
  • 02:46In-capillary Protein Measurement Sample Preparation and Signal Protein Analysis
  • 06:32Results: Representative Signaling Protein Marker Expression in Primed and Unprimed Neonatal Rat Brain Tissue
  • 08:26Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol to isolate brain nuclei in the neonatal rat brain in conjunction with first colostrum feeding. This technique allows the study of nutrient insufficiency stress in the brain as modulated by enterocyte signaling.

Tags

OxytocinBrain NucleiNeonatal RatColostrum FeedingCellular StressInflammationPost-natal DevelopmentMilk SucklingBrain PunchesProtein ExtractionProtein Measurement

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Seven Steps to Stellate Cells

Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Culturing Microglia from the Neonatal and Adult Central Nervous System

Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Read Article